Pepsinogen II (PG II)

Anti- PG II Antibody (Datasheet)

Source	Mouse monoclonal antibody，cultured in vitro
Platforms	Immunofluorescence, Chemiluminescence, Colloidal gold and Immunological latex turbidimetry
Catalog number	K28c9	K30t2	K101h3
Applications	Capture	Detection	Capture
Buffer	1x PBS
Purity	Purity>98%，purified by Protein A/G chromatography
Storage instructions	Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

PG II protein ( Datasheet)

Description	PG II protein (41.4kD) with His-tag, cultured in vitro
Applications	Calibrator and quality control product
Catalog number	K3926
Source	Mammalian cell
Purity	>90% as analysized by SDS-PAGE
Buffer	1 x PBS，pH 7.4
Storage instructions	Store at 2 - 8°C (35.6 – 46.4°F) for up to 3 years.
Sequence	AVVKVPLKKF KSIRETMKEK GLLGEFLRTH KYDPAWKYRF GDLSVTYEPM AYMDAAYFGE ISIGTPPQNF LVLFDTGSSN LWVPSVYCQS QACTSHSRFN PSESSTYSTN GQTFSLQYGS GSLTGFFGYD TLTVQSIQVP NQEFGLSENE PGTNFVYAQF DGIMGLAYPA LSVDEATTAM QGMVQEGALT SPVFSVYLSN QQGSSGGAVV FGGVDSSLYT GQIYWAPVTQ ELYWQIGIEE FLIGGQASGW CSEGCQAIVD TGTSLLTVPQ QYMSALLQAT GAQEDEYGQF LVNCNSIQNL PSLTFIINGV EFPLPPSSYI LSNNGYCTVG VEPTYLSSQN GQPLWILGDV FLRSYYSVYD LGNNRVGFAT AAHHHHHH
SDS-PAGE

Antibody Product Information

Antibody Evaluation (Immunofluorescence-based)

Immunofluorescence-Based test strip was prepared with K28c9-K30t2 antibody (30 Abbott calibrators, Concentration: 4-100 ng/ml, R² >98%)

Fig. 1 Clinical sample analysis

Accuracy
The quality control samples with concentration of 15 ng/ml-20 ng/ml and 45 ng/ml-55 ng/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)

	Sample (ng/ml)	Test 1	Test 2	Test 3	Average (ng/ml)	RSD	Standard
Batch 1	17.32	17.32	16.98	18.34	17.55	1.31%	±15%
Batch 1	49.56	48.39	44.82	49.31	47.51	-4.14%
Batch 2	17.32	16.23	15.75	17.36	16.45	-5.04%
Batch 2	49.56	49.33	47.6	48.70	48.54	-2.05%
Batch 3	17.32	17.22	16.71	16.02	16.65	-3.87%
Batch 3	49.56	47.71	51.41	51.75	50.29	1.47%

Repeatability
The quality control samples with concentration of 25 ng/ml-30 ng/ml and 70 ng/ml-80 ng/ml respectively were required to repeat test over 10 times. CV= (S / average

) ×100% (CV < 15% as indicated in the following table)

	Low Value （17.32ng/ml）	High Value （49.56ng/ml）
Test 1	18.83	50.60
Test 2	16.63	48.22
Test 3	18.62	44.81
Test 4	18.46	53.38
Test 5	17.25	50.75
Test 6	15.73	51.48
Test 7	18.24	52.40
Test 8	18.25	49.70
Test 9	18.54	45.39
Test 10	15.87	45.43
Average(ng/ml)	17.64	49.22
SD	1.18	3.10
CV	6.69%	6.30%
Standard	±15%

Antibody Evaluation (Immunological Turbidimetry)

Fig. 2 Calibration curve

R1: Buffer
R2: Combination of K28c9-K30t2 matched pair antibody and latex particals
Sample: PG II calibrator
Equipment: Hitachi 7080

Linear range

Linear dependence > 99% with concentration between 0-100 ng/ml.

Theoretical value (ng/ml)	Measured value 1	Measured value 2	Average
0.00	2.1	1.54	1.82
12.50	11.22	12.02	11.62
25.00	25.8	24.8	25.3
50.00	48.25	47.36	47.805
100.00	98.76	96.74	97.75

Fig. 3 Linearity range

Antibody Evaluation (Immunological Turbidimetry)

Fig. 4 Calibration curve

R1: Latex particals
R2: K28c9-K30t2 matched pair antibody
Sample: PG II calibrator
Equipment: Hitachi 7080

Description

Pepsinogen I, the precursors of pepsin, is produced by the gastric mucosa and released into the gastric lumen and peripheral circulation. Pepsinogen consists of a single polypeptide chain of 375 amino acids with an average molecular weight of 42 kD. PG I (isoenzyme 1-5) is secreted mainly by chief cells in the fundic mucosa, whereas PG II (isoenzyme 6-7) is secreted by the pyloric glands and the proximal duodenal mucosa.

Precursor reflects numbers of stomach surface cells as well as glandular cells, and monitors gastric atrophy indirectly. They are also extraordinarily stable because they perform their jobs under the harsh conditions present in the digestive system. Advanced gastric atrophy and hypo- or achlorhydrias cause very low levels of pepsinogen I in contrast to pepsinogen II, which remains elevated in serum. Serum pepsinogen II indicates function and states of gastric mucosa.

Pepsinogen II (PG II)

Antibody Evaluation (Immunofluorescence-based)

Antibody Evaluation (Immunological Turbidimetry)

Linear range

Antibody Evaluation (Immunological Turbidimetry)

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